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Individual fish were divided using a razor blade into 0.5 cm sections and added to a 40 mL glass tube along with 0.1 mL of DI water (18 M-cm Milli-Q, EMD Millipore, Burlington, MA) whereupon tissues were homogenized into a paste using an Omni TH (Omni Int., Kennesaw, GA) tissue homogenizer

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The most common patient-reported effects with supervised Epithalon use are mild and transient: occasional mild injection-site reactions during the 10-day loading cycle, changes in sleep timing that usually resolve as the cycle completes, and occasional fatigue during the loading phase

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